Robustness of tissue organization and cell structure against gravity or physical force – Publicly Invited Research 2018－2019

  1. A01 Ogura
  2. A01 H. Takahashi
  3. A01 S. Takahashi
  4. A01 Michiue
  5. A01 Hinoi
  6. A01 Tsumoto
  7. A01 Nikawa
  8. A01 Chatani
  9. A01 Kawakami
  10. A01 Akiyama
  11. A01 Tomita
  1. A02 Shinohara
  2. A02 Mieda
  3. A02 Maekawa
  4. A02 Abe
  5. A02 Ohgami
  6. A02 Kawano
  7. A02 Takano
  1. A03 Suzuki
  2. A03 Nakamura
  3. A03 Harada
  4. A03 Kobayashi
  5. A03 Miyamoto
  6. A03 Funayama
  7. A03 Kakinuma
  1. B01 Lazarus
  2. B01 Kato
  3. B01 Kunieda
  4. B01 Kitaya
  5. B01 Sawano
Research Subject Robustness of tissue organization and cell structure against gravity or physical force
Research Group Leader
name

The homeostasis of cells, tissues and organisms is constituted on the basis of our living on earth. Therefore, the change of gravity as well as external pressure will lead to the severe effects on the biological events. From this viewpoint, it will be a great merit for human if we can acquire the technique of resisting from such external pressure and gravity. Cells respond to physical forces, triggering the change of not only cytoskeleton structure but also cell shape. However, it has not been well understood that the mechanism by which the organization maintains the structural robustness against external pressure. In this study, we investigate how our tissue has its own robustness against physical environmental changes in embryos. In detail,

1) Verification of the change of the localization of cytoskeleton/cytoskeleton binding protein according to physical force

We observe the time course of the localization of cytoskeleton or cytoskeletal binding protein when applying physical force. Also, we analyze the change of cell shape.

2) Robustness of tissue deformation against physical force by ectopic expression of cytoskeleton / cytoskeletal binding protein

Physical pressure is applied to an embryo ectopically expressing cytoskeleton and cytoskeleton binding protein in cells, and we observe the change of the effects.

3) The effect of vitelline membrane against physical force

We observe the difference of actin orientation and cell shape between a membrane-covered embryo and a naked embryo. From the analysis, we want to show the role of vitelline membrane against physical force.